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What is eosin-Nigrosin stain?

What is eosin-Nigrosin stain?

Eosin-Nigrosin is a staining technique that assesses the vitality of a sperm sample when the initial motility is less than 25 %. Nigrosin increases the contrast between the background and sperm heads, making sperm easier to visualize.

How do you make eosin-Nigrosin stain?

Mix 1 drop of semen with 2 drops of eosin solution. Wait 30 s and add 3 drops of 100 g/L of nigrosin solution. Mix and make smear; let air dry.

Why is eosin stain used on sperm?

Eosin- Nigrosin is a staining technique that assesses the vital- ity of a sperm sample when the initial motility is less than 25 % [ 1– 3]. Nigrosin increases the contrast between the background and sperm heads, making the sperm easier to visualize.

Which stain is used for sperm morphology?

Eosin-nigrosin is the most commonly used staining method in sperm morphological analysis. It allows detection of live and dead sperm cells (8), and is recommended by the WHO for human semen analysis.

How do you make eosin dye?

Preparation – Dissolve eosin in water and then add this to 95% alcohol (one part eosin solution with 4 parts alcohol). To the final mixture add a few drops of acetic acid (0.4ml). The acetic acid increases the staining intensity of eosin.

What is the purpose of nigrosin?

The nigrosin component of the stain is used as a counterstain to ease visualization of the unstained live spermatozoa. This stain is also widely used to assess the morphologic characteristics of the spermatozoa. Preparations are made by depositing one drop of the supravital eosin-nigrosin stain on a prewarmed slide.

What is nigrosin stain used for?

Nigrosin is a simple and indirect stain used for determining bacterial morphology. The shapes and sizes of the organisms are seen as color-free outlines against the dark background. An advantage of using this method is that prior fixation by heat is not needed, so the organisms are seen in more lifelike shapes.

How do you make a nigrosin solution?

Nigrosin: Add 10 g Nicrosin (C.C.) to 100 ml distilled water and dissolve by placing in a boiling water bath for 30 minutes. Replace water lost by evaporation and add 0.5 ml of formalin.

How do you make nigrosin dye?

In staining dyes, nigrosin (CI 50415, Solvent black 5) is a mixture of black synthetic dyes made by heating a mixture of nitrobenzene, aniline, and hydrochloric acid in the presence of copper or iron.

Is nigrosin a simple stain?