How can microarray detect cancer?
Microarray technology provides a method for monitoring the RNA expression levels of many thousands of genes simultaneously in primary tumours and cell lines. In principle, data from such studies could revolutionise cancer diagnosis.
What do microarrays measure?
A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. DNA microarrays are microscope slides that are printed with thousands of tiny spots in defined positions, with each spot containing a known DNA sequence or gene.
What are the types of microarrays?
Types of microarrays include:
- DNA microarrays, such as cDNA microarrays, oligonucleotide microarrays, BAC microarrays and SNP microarrays.
- MMChips, for surveillance of microRNA populations.
- Protein microarrays.
- Peptide microarrays, for detailed analyses or optimization of protein–protein interactions.
- Tissue microarrays.
How microarray is a powerful genomic tool for research in clinical diagnostics?
Initially designed to measure gene transcriptional levels, microarray technologies are now used for comparing other genome features among individuals and their tissues and cells. Results provide valuable information on disease subcategories, disease prognosis, and treatment outcome.
Is microarray a diagnosis?
For instance, microarrays are currently a key tool in genetic diagnosis, allowing doctors to identify specific subtypes within an overall disease category based on differences in gene expression.
Which of the following results can a DNA microarray assay detect or identify?
Which of the following results can a DNA microarray assay detect or identify? They allow the expression of many or even all of the genes in a genome to be compared at once. Researchers are looking for better treatments for breast cancer.
Why is DNA microarray an important tool?
DNA microarray is an effective tool in transcriptomics that helps us in studying and analyzing the mRNA expression of almost every gene present in an organism. With the availability of whole-genome sequencing of microorganisms, it has now become possible to identify the genes with potential for bioremediation.
What is an advantage of RNA Seq over microarrays?
The advantage of RNA-Seq over microarrays is that it provides an unbiased insight into all transcripts (Zhao et al., 2014). Thus, RNA-Seq is generally reliable for accurately measuring gene expression level changes.
What are the steps of microarray analysis?
Steps involved in cDNA based microarray:
- Sample collection.
- Isolation of mRNA.
- Creation of labeled cDNA.
- Hybridization.
- Collection and analysis.
What is application of microarray?
The current scope of microarray applications includes sequencing by hybridization, resequencing, mutation detection, assessment of gene copy number, comparative genome hybridization, drug discovery, expression analysis, and immunoassay (protein microarrays).
Why do we use microarrays?
Microarrays can also be used to study the extent to which certain genes are turned on or off in cells and tissues. In this case, instead of isolating DNA from the samples, RNA (which is a transcript of the DNA) is isolated and measured. Today, DNA microarrays are used in clinical diagnostic tests for some diseases.