What is bactec Mgit?
The BACTEC MGIT 960 instrument is a fully automated system that exploits the fluorescence of an oxygen sensor to detect growth of mycobacteria in culture. Its performance was compared to those of the radiometric BACTEC 460 instrument and egg-based Lowenstein-Jensen medium.
What is Mgit test?
MGIT stands for Mycobacteria Growth Indicator Tube. It is a system that determines whether or not TB bacteria will grow in the presence of TB drugs. If the bacteria grow, then they are resistant to the drugs, because the drugs could not stop their growth.
What is bactec?
The BD BACTEC™ Automated Blood Culture System utilizes fluorescent technology in detecting the growth of organisms in the blood culture bottles. The sample to be tested is inoculated into one or more vials which are inserted into the BD Bactec fluorescent series instrument for incubation and periodic reading.
What is mean by AFB?
Acid-fast bacillus (AFB) is a type of bacteria that causes tuberculosis and certain other infections. Tuberculosis, commonly known as TB, is a serious bacterial infection that mainly affects the lungs. It can also affect other parts of the body, including the brain, spine, and kidneys.
How is MGIT test done?
The instrument scans the MGIT every 60 minutes for increased fluorescence. Analysis of the fluorescence is used to determine if the tube is instrument positive; i.e., the test sample contains viable organisms. An instrument-positive tube contains approximately 105 to 106 colony-forming units per milliliter (CFU/mL).
What is LPA in TB?
The line probe assay (LPA), based on strip technology was used to diagnose TB and detect RIF as well as Isoniazid (INH) resistance due to mutations in rpoβ, and both inhA and katG genes.
How does the bactec work?
The BD BACTEC™ Automated Blood Culture System utilizes fluorescent technology in detecting the growth of or- ganisms in the blood culture bottles. When microorganisms are present in the cultured vials, they metabolize nutrients in the culture medium, releasing carbon dioxide into the medi- um.