What is a HPLC system?
High Performance Liquid Chromatography (HPLC) is a form of column chromatography that pumps a sample mixture or analyte in a solvent (known as the mobile phase) at high pressure through a column with chromatographic packing material (stationary phase).
What are the types of HPLC?
- Partition chromatography.
- Normal–phase chromatography.
- Displacement chromatography.
- Reversed-phase chromatography (RPC)
- Size-exclusion chromatography.
- Ion-exchange chromatography.
- Bioaffinity chromatography.
- Aqueous normal-phase chromatography.
Why HPLC is used?
HPLC is the form of liquid chromatography that is generally used in the pharmaceutical industry, as it can provide the precise results that are required. The results can be used to analyse finished drug products and their ingredients quantitatively and qualitatively during the manufacturing process.
Why HPLC is done?
The main purpose of the HPLC technique is to identify, quantify and purify a particular analyte or compound. Both quantitative and qualitative analysis can be done.
Which columns are used in HPLC?
Different Types of HPLC Columns Used in Analysis
- Normal Phase Columns.
- Reverse Phase Columns.
- Ion Exchange Columns.
- Size Exclusion Columns.
What is the difference between UV and HPLC?
There was no statistically significant difference between the mean values, although UV method showed slightly higher % R.S.D. value compared with HPLC method. Therefore, both analytical methods, were found to be accurate, precise and could be used for routine quality control analysis of repaglinide.
Why HPLC is more sensitive than UV?
HPLC-UV is GENERALLY much better than UV alone. Samples may be contaminated with other chemicals that absorb at the same wavelength to give a falsely high concentration of desired analyte in a UV protocol.