What is CD14 FITC?
CD14 has been identified as a high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS) and serum LPS-binding protein, LPB. 557153 Rev. 6. FITC. Fluorescein (FITC) is part of the BD blue family of dyes.
What is CD14 gene?
CD14 (cluster of differentiation 14) is a human protein made mostly by macrophages as part of the innate immune system. It helps to detect bacteria in the body by binding lipopolysaccharide (LPS), a pathogen-associated molecular pattern (PAMP).
Do all monocytes express CD14?
The most predominant of the three, termed “classical monocytes”, express high levels of CD14 on their surface, are devoid of surface CD16 and account for approximately 80% of the total monocyte population. The remaining 20% express CD16 and have been further classified into two subtypes.
What is soluble CD14?
Soluble CD14 is associated with morbidity and mortality in HIV disease. It is a co-receptor for lipopolysaccharide (LPS) that is released from monocytes upon activation.
Is CD14 a macrophage marker?
Abstract. In the past, CD14 has been viewed simply as a useful marker molecule for monocytes and macrophages. Now, new findings on its role in binding of LPS-LBP complexes and in signal transduction have engendered renewed interest in the properties of CD14.
Is CD14 a toll-like receptor?
CD14 is a glycosylphosphatidylinositol (GPI)-anchored receptor known to serve as a co-receptor for several Toll-like Receptors (TLRs) both at the cell surface and in the endosomal compartment. CD14 can be expressed by cells of both hematopoietic and non-hematopoietic origin as a cell membrane or secreted protein.
Do B cells express CD14?
These cells produced IL-6 in response to lipopolysaccharide, and this response was blocked by anti-CD14 antiserum. Thus, our findings demonstrated that human B cells can express functionally active CD14.
What is CD14 cells?
CD14 is a glycolipid-anchored membrane glycoprotein expressed on cells of the myelomonocyte lineage including monocytes, macrophages, and some granulocytes. CD14 is a key molecule in the activation of innate immune cells and exists as a membrane-anchored or soluble form.
Is CD14 a TLR4?
The most important of the CD14 co-receptors is TLR4. TLRs are members of the interleukin 1 receptor (IL-1R) family,31,32 an evolutionary conserved signalling system that is a critical determinant of the innate immune and inflammatory responses against invading pathogens.